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    §Weight LossResearch protocol

    Survodutide.

    Survodutide (BI 456906) is an investigational dual GLP‑1/glucagon receptor agonist under development for obesity and metabolic liver disease[1][2]. In phase 2 trials, once‑weekly subcutaneous dosin...

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    Research Use Only. PeptiJournal supports private research documentation and calculation support. It does not provide medical advice, human-use directions, or claims of safety or effectiveness.

    Calculated-volume support unavailable

    This selected cited guide does not contain one unambiguous vial, per-event amount, cadence, and diluent set. No value was inferred from general library metadata. Review the cited table below before creating a private Research Use Only record.

    Cited protocol & reconstitution guide

    Source-backed reference fields by phase, including any volume fields authored in the cited guide.

    46 weeks (0.6 mg arm)

    0.6 mg

    Units / volume12 units (0.12 mL)

    46 weeks (2.4 mg arm)

    2.4 mg

    Units / volume48 units (0.48 mL)

    46 weeks (3.6 mg arm)

    3.6 mg

    Units / volume72 units (0.72 mL)

    46 weeks (4.8 mg arm)

    4.8 mg

    Units / volume96 units (0.96 mL)

    Weeks 0–20 (Dose-increase phase)

    Escalating to assigned arm

    Units / volume—

    Weeks 20–46 (Steady-dose phase)

    Assigned arm dose held steady

    Units / volume—

    76 weeks (Phase 3 SYNCHRONIZE-1 — extended exposure)

    Not stated as a fixed mg value in the topline release

    Units / volume—

    Overview

    Overview

    Survodutide (BI 456906) is an investigational dual GLP‑1/glucagon receptor agonist under development for obesity and metabolic liver disease[1][2]. In phase 2 trials, once‑weekly subcutaneous dosing (0.6–4.8 mg) produced dose‑dependent weight loss of 12.5–14.9% versus placebo over 46 weeks[1]. This educational protocol presents a once‑weekly subcutaneous titration approach using a practical dilution for clear insulin‑syringe measurements. Reconstitute: Add 2.0 mL bacteriostatic water → 5 mg/mL

    Category
    Weight Loss
    Routes
    subcutaneous

    Mechanism

    Survodutide

    Mechanism of action

    Mechanism of action

    Survodutide acts on two targets at the same time: the GLP-1 receptor and the glucagon receptor . That is why it is grouped with newer obesity peptides like tirzepatide and retatrutide, but it is not the same as either one. In plain English: GLP-1 may lower appetite and slow how fast food leaves the stomach. Glucagon may raise energy use , meaning the body may burn more calories at rest. Glucagon also acts in the liver, which is why Survodutide is being studied for MASH. In research papers, Survodutide is called a dual agonist because one peptide molecule turns on both receptors. It also has a fatty-acid tail, similar to semaglutide, which helps it last long enough for once-weekly dosing. In Phase 2, around 16.6% of survodutide participants reported decreased appetite vs 3.4% on placebo. Preclinical work showed Survodutide raised energy use in animal models and lowered liver fat. Glucagon receptors are common in the liver, which is why Survodutide is also being tested in MASH and MASH-cirrhosis trials.

    Key research findings
    • 01

      Survodutide (BI 456906) is a once-weekly dual glucagon (GCG) and GLP-1 receptor agonist (mechanistic / human study).

    • 02

      In Phase 2 obesity research, survodutide produced dose-dependent body-weight reduction (human study, Phase 2).

    • 03

      Phase 3 SYNCHRONIZE obesity trials reported positive primary-endpoint results, with substantial reductions in liver fat in associated analyses (human study, Phase 3; SYNCHRONIZE-1 published in the New England Journal of Medicine, 2025).

    • 04

      The compound is also studied in metabolic dysfunction-associated steatohepatitis (MASH) research (LIVERAGE Phase 3 program) and received FDA Breakthrough Therapy designation in that research context in 2024 (human study; regulatory designation).

    • 05

      Gastrointestinal effects were the most commonly observed effects in research (human study observation).

    Primary source: Phase 3 obesity (SYNCHRONIZE-1): 725 adults, 76 weeks, average 16.6% weight loss vs 3.2% placebo. 85.1% reached ≥5% loss. Topline reported by Boehringer Ingelheim, April 28, 2026. Phase 2 obesity (Le Roux et al., Lancet Diabetes & Endocrinology, 2024): 387 adults, 46 weeks, with more weight loss at higher weekly doses. The 4.8 mg arm reached around 14.9% loss in the main analysis, or about 18.7% among people who completed the trial. Phase 2 MASH (NEJM, 2024): 295 adults with MASH and liver scarring. More people on Survodutide improved than on placebo, but stomach and gut observed effects were common. Phase 2 type 2 diabetes (Blüher et al., Diabetologia, 2023): Compared to placebo and open-label semaglutide. Survodutide reduced HbA1c by ~1.5% versus placebo, comparable to 1.0 mg/week semaglutide. Systematic review and meta-analysis (PMC, 2025): A pooled review of randomized Survodutide trials in obesity. It supported the dose-related weight-loss finding and the stomach-and-gut observed effect pattern.

    Pharmacokinetic profile

    Literature reference (RUO)

    Single-dose plasma curve over 24h. Shaded band = commonly-cited therapeutic window. Illustrative only.

    Researched Effects

    Researched benefits

    Areas of active research and investigation. Results may vary and are based on preclinical or early clinical data.

    ✨

    Dose‑dependent weight loss of 12.5–14.9% (vs placebo) over 46 weeks in phase 2 obesity trials[1].

    ✨

    Improvements in liver‑related biomarkers in NAFLD/NASH populations[2].

    ✨

    Meta‑analysis confirms consistent weight‑loss efficacy across multiple randomized controlled trials[5].

    Protocol Reference

    Protocol reference

    Research Use Only. PeptiJournal supports private research documentation and calculation support. It does not provide medical advice, human-use directions, or claims of safety or effectiveness.
    subcutaneous

    Commonly cited research range: 0.6–6 mg, weekly.

    Reference figures reported in the research literature — not a dosing recommendation. For interactive vial math and scheduling, see the Calculator and Schedule tabs.

    Cited protocol & reconstitution guide

    Source-backed reference fields by phase, including any volume fields authored in the cited guide.

    46 weeks (0.6 mg arm)

    0.6 mg

    Units / volume12 units (0.12 mL)

    46 weeks (2.4 mg arm)

    2.4 mg

    Units / volume48 units (0.48 mL)

    46 weeks (3.6 mg arm)

    3.6 mg

    Units / volume72 units (0.72 mL)

    46 weeks (4.8 mg arm)

    4.8 mg

    Units / volume96 units (0.96 mL)

    Weeks 0–20 (Dose-increase phase)

    Escalating to assigned arm

    Units / volume—

    Weeks 20–46 (Steady-dose phase)

    Assigned arm dose held steady

    Units / volume—

    76 weeks (Phase 3 SYNCHRONIZE-1 — extended exposure)

    Not stated as a fixed mg value in the topline release

    Units / volume—

    Titration protocol

    1. 46 weeksStart
      0.6 mg

      Lowest studied dose. Once weekly, subcutaneous. Rotate injection sites.

    2. 46 weeksBuild
      2.4 mg

      Mid dose. Once weekly, subcutaneous. Rotate injection sites.

    3. 46 weeksBuild
      3.6 mg

      Mid-high dose. Once weekly, subcutaneous. Rotate injection sites.

    4. 46 weeksBuild
      4.8 mg

      Top dose studied. Once weekly, subcutaneous. Rotate injection sites.

    5. Weeks 0–20Build
      Escalating to assigned arm

      The dose went up during the first 20 weeks. Once weekly.

    6. Weeks 20–46Build
      Assigned arm dose held steady

      Dose stayed steady for the next 26 weeks — hold, do not increase further. Once weekly.

    7. 76 weeksMaintenance
      Not stated as a fixed mg value in the topline release

      Phase 3 SYNCHRONIZE-1. The dose increase was slower and more flexible than in Phase 2. Once weekly.

    Storage & Handling

    Storage requirements(typical for most peptides)

    ❄️
    Lyophilized (powder)
    -20°C (frozen)

    Can be stored for extended periods. Protect from moisture.

    🧊
    Reconstituted
    2-8°C (refrigerated)

    Store in refrigerator door. Never freeze after reconstitution.

    ⏱️
    Stability window
    28-30 days after reconstitution

    Label vials with reconstitution date. Discard if cloudy.

    Reconstitution steps

    1. 01🌡️01 Wipe the vial top — Use a fresh alcohol swab on the rubber stopper of both the survodutide vial and the BAC water vial.
    2. 02🧴Draw 2 mL bacteriostatic water into a sterile syringe — this 10 mg vial yields 5 mg/mL.
    3. 03💉03 Add slowly — Insert the needle and let the water run down the inside wall of the survodutide vial, not directly onto the powder.
    4. 04💧04 Swirl, do not shake — Gently swirl or roll the vial between your hands until the powder dissolves into a clear solution. Shaking can damage the peptide.
    5. 05🔄05 Inspect — The solution should look clear with no cloudiness or particles. If it looks off, do not use the vial.
    6. 06🏷️06 Label and refrigerate — Write the reconstitution date on the vial. Store at 2 to 8 °C (35.6 to 46.4 °F).
    7. 07❄️08 Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

    Additional storage notes

    Dry powder (sealed vial, long-term)

    -4 °F (-20 °C) or colder — Best for long storage. Keep dark and dry.

    Dry powder (short-term)

    35.6 to 46.4 °F (2 to 8 °C) — Acceptable for short transit and short holds.

    Reconstituted (in BAC water)

    35.6 to 46.4 °F (2 to 8 °C) — Refrigerate. Use within the BAC water shelf window.

    Appearance

    Clear after mixing — Cloudy or particulate solution means stop using it.

    Clinical Evidence

    Clinical evidence

    Phase 2 clinical trials report body-weight reduction and metabolic-liver research endpoints; it remains investigational.

    Phase 3 obesity (SYNCHRONIZE-1): 725 adults, 76 weeks, average 16.6% weight loss vs 3.2% placebo. 85.1% reached ≥5% loss. Topline reported by Boehringer Ingelheim, April 28, 2026. Phase 2 obesity (Le Roux et al., Lancet Diabetes & Endocrinology, 2024): 387 adults, 46 weeks, with more weight loss at higher weekly doses. The 4.8 mg arm reached around 14.9% loss in the main analysis, or about 18.7% among people who completed the trial. Phase 2 MASH (NEJM, 2024): 295 adults with MASH and liver scarring. More people on Survodutide improved than on placebo, but stomach and gut observed effects were common. Phase 2 type 2 diabetes (Blüher et al., Diabetologia, 2023): Compared to placebo and open-label semaglutide. Survodutide reduced HbA1c by ~1.5% versus placebo, comparable to 1.0 mg/week semaglutide. Systematic review and meta-analysis (PMC, 2025): A pooled review of randomized Survodutide trials in obesity. It supported the dose-related weight-loss finding and the stomach-and-gut observed effect pattern.

    1. 01Survodutide (BI 456906) is a once-weekly dual glucagon (GCG) and GLP-1 receptor agonist (mechanistic / human study).
    2. 02In Phase 2 obesity research, survodutide produced dose-dependent body-weight reduction (human study, Phase 2).
    3. 03Phase 3 SYNCHRONIZE obesity trials reported positive primary-endpoint results, with substantial reductions in liver fat in associated analyses (human study, Phase 3; SYNCHRONIZE-1 published in the New England Journal of Medicine, 2025).
    4. 04The compound is also studied in metabolic dysfunction-associated steatohepatitis (MASH) research (LIVERAGE Phase 3 program) and received FDA Breakthrough Therapy designation in that research context in 2024 (human study; regulatory designation).
    5. 05Gastrointestinal effects were the most commonly observed effects in research (human study observation).

    Evidence maturity varies by compound; much peptide research is preclinical (in vitro or animal-model). Where human data are limited, findings should be read as research observations, not clinical conclusions.

    References

    Literature references

    Published research articles and sources related to Survodutide.

    1. 01
      Boehringer Ingelheim. Results from Phase III SYNCHRONIZE-1 obesity trial (press release, April 28, 2026). Boehringer Ingelheim (2026)
      et al. (2026)
    2. 02
      Le Roux CW, Steen O, Lucas KJ, Startseva E, Unseld A, Hennige AM. Glucagon and GLP-1 receptor dual agonist survodutide for obesity: a randomised, double-blind, placebo-controlled, dose-finding phase 2 trial. The Lancet Diabetes & Endocrinology (2024)
      et al. (2024)
    3. 03
      Sanyal AJ, Bedossa P, Fraessdorf M, et al. A Phase 2 Randomized Trial of Survodutide in MASH and Fibrosis. New England Journal of Medicine (2024)
      et al. (2024)
    4. 04
      Blüher M, Rosenstock J, Hoefler J, Manuel R, Hennige AM. Dose-response effects on HbA1c and bodyweight reductions with a dual glucagon/GLP-1 receptor agonist, survodutide, compared with placebo and open-label semaglutide in people with type 2 diabetes: a randomised clinical trial. Diabetologia (2024)
      et al. (2024)
    5. 05
      Zimmermann T, Thomas L, Baader-Pagler T, et al. BI 456906: Discovery and preclinical pharmacology of a novel GCGR/GLP-1R dual agonist with robust anti-obesity efficacy. Molecular Metabolism (2022)
      et al. (2022)
    6. 06
      Authors et al. Evaluating the efficacy and safety of survodutide for obesity: a systematic review and meta-analysis of randomized controlled trials. PMC (peer-reviewed meta-analysis) (2025)
      et al. (2025)
    7. 07
      Mikhail N, Wali S. Survodutide, a promising agent with novel mechanism of action for treatment of obesity and type 2 diabetes. Journal of Endocrinology and Disorders (2024)
      et al. (2024)
    8. 08
      ClinicalTrials.gov. A Study to Test How Well Different Doses of BI 456906 Help People With Overweight or Obesity Lose Weight (NCT04667377, Phase 2). ClinicalTrials.gov (2024)
      et al. (2024)
    9. 09
      ClinicalTrials.gov. A Study to Test Whether Survodutide (BI 456906) Helps People Living With Overweight or Obesity Lose Weight (SYNCHRONIZE-1, NCT06066529). ClinicalTrials.gov (2026)
      et al. (2026)
    10. 10
      U.S. Food and Drug Administration. Drugs@FDA: search for survodutide approval status (no approved product as of June 2026). FDA.gov (2026)
      et al. (2026)
    Search PubMed for Survodutide

    Observed Effects

    Observed effects in cited research

    Phase 2 obesity trial reported rates
    • Any stomach or gut observed effect: 75% on Survodutide vs 42% on placebo .
    • Stopped because of observed effects: 25% on Survodutide vs 4% on placebo . This was mostly during the fast 20-week dose increase.
    • Serious observed effects: 4.2% on Survodutide vs 6.5% on placebo .
    • Notable single events: one case of dehydration with kidney failure and one case of angioedema, a serious swelling reaction.
    Phase 2 MASH trial reported rates
    • Nausea: 66% on survodutide vs 23% on placebo .
    • Diarrhea: 49% on survodutide vs 23% on placebo .
    • Vomiting: 41% on survodutide vs 4% on placebo .
    • Stopped because of observed effects: 20% on Survodutide vs 3% on placebo . Most were stomach and gut observed effects during dose increase.
    Phase 3 SYNCHRONIZE-1 safety signal
    • Boehringer reported that stomach and gut observed effects in SYNCHRONIZE-1 were mild to moderate and most common while the dose was going up. No new safety concerns were reported in the topline release. Full safety data is due at ADA 2026 in June.
    GLP-1 class cautions
    • Pancreatitis: GLP-1 drugs carry a pancreas inflammation warning.
    • Medullary thyroid cancer: GLP-1 drugs carry a thyroid cancer warning based on animal data.
    • Heart rate: a small heart-rate increase was reported with survodutide.
    • Hypoglycemia: higher risk if combined with insulin or sulfonylureas.

    Research Considerations

    Research considerations

    Research Use Only - not for human or veterinary therapeutic use. Investigational compound currently in clinical development; not approved for general use. Consult a licensed healthcare professional for any clinical decisions.

    • Pregnancy and breastfeeding: not studied in these groups. Avoid.
    • Personal or family history of medullary thyroid cancer or MEN 2: this is a common warning for GLP-1 drugs.
    • History of pancreatitis: GLP-1 drugs carry a pancreas inflammation warning.
    • Severe stomach or gut disease: nausea, vomiting, diarrhea, and constipation are common with Survodutide.
    • Diabetes treated with insulin or sulfonylureas: blood sugar may need close medical adjustment.
    • Children and teenagers: no pediatric data exists.
    • Known peptide allergies: one Phase 2 participant had angioedema, a serious swelling reaction.

    Factors noted in the research literature; not patient-specific medical advice.

    Regulatory Status

    Regulatory status

    RUO

    Research Use Only. PeptiJournal supports private research documentation and calculation support. It does not provide medical advice, human-use directions, or claims of safety or effectiveness.

    Comparisons

    Comparisons

    CompoundMechanismRouteStatus
    SurvodutidethisAn investigational long-acting dual agonist of GLP-1 and glucagon receptors studied for appetite regulation and increased energy expenditure.subcutaneousInvestigational / RUO
    TirzepatideActivates both GIP and GLP-1 receptors, providing synergistic effects on insulin secretion, glucose control, and appetite regulation.subcutaneousInvestigational / RUO
    5-Amino-1MQInhibits nicotinamide N-methyltransferase (NNMT), increasing NAD+ availability and enhancing mitochondrial metabolism.oral, subcutaneousInvestigational / RUO
    AdipotideA peptidomimetic that targets prohibitin on the vasculature of white adipose tissue and delivers a pro-apoptotic sequence, studied for selective reduction of fat-tissue blood supply.subcutaneousInvestigational / RUO
    AICARA cell-permeable nucleoside phosphorylated intracellularly to ZMP, an AMP-mimetic that activates AMP-activated protein kinase (AMPK), shifting cells toward oxidative metabolism.subcutaneousInvestigational / RUO
    TB-500Upregulates actin, promotes cell migration, reduces inflammation, and stimulates wound healing and tissue repair.subcutaneous, intramuscularInvestigational / RUO
    TesamorelinBinds to GHRH receptors to stimulate endogenous GH production. Preferentially reduces abdominal fat accumulation.subcutaneousInvestigational / RUO

    Attributes shown for research comparison only; not a statement of efficacy or therapeutic equivalence.

    FAQ

    Frequently asked questions

    Research-use notice

    Research Use Only. This educational content and calculation support is intended for private research documentation. It does not provide medical advice, human-use directions, or claims of safety or effectiveness.

    Cited guide source: View source

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